3N44
Crystal structure of the mature envelope glycoprotein complex (trypsin cleavage; Osmium soak) of Chikungunya virus.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-10-29 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.089, 89.954, 174.018 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.300 - 2.350 |
R-factor | 0.231 |
Rwork | 0.229 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | P62E1 ENVELOPE GLYCOPROTEINS FROM CHIKUNGUNYA VIRUS |
RMSD bond length | 0.007 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.9.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.480 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 35477 | |
<I/σ(I)> | 29.5 | 4.6 |
Completeness [%] | 91.5 | 62.2 |
Redundancy | 7 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | 8-12% PEG4K, 100mM NaAcetate, 100mM Hepes pH7.0 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |