3N42
Crystal structures of the mature envelope glycoprotein complex (furin cleavage) of Chikungunya virus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-11-13 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.391, 90.678, 178.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.600 - 3.000 |
R-factor | 0.249 |
Rwork | 0.247 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | P62E1 ENVELOPE GLYCOPROTEINS FROM CHIKUNGUNYA VIRUS |
RMSD bond length | 0.007 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.9.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.700 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 18964 | |
<I/σ(I)> | 9.2 | 2 |
Completeness [%] | 96.4 | 82.2 |
Redundancy | 3.7 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | 8-12% PEG4K, 100mM NaAcetate, 100mM Hepes pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |