3N41
Crystal structure of the mature envelope glycoprotein complex (spontaneous cleavage) of Chikungunya virus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2009-08-08 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.240, 99.610, 107.100 |
Unit cell angles | 90.00, 102.90, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.010 |
R-factor | 0.253 |
Rwork | 0.252 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | P62E1 ENVELOPE GLYCOPROTEINS FROM CHIKUNGUNYA VIRUS |
RMSD bond length | 0.007 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.9.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.170 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 20262 | |
<I/σ(I)> | 12.2 | 2 |
Completeness [%] | 98.6 | 94.8 |
Redundancy | 3.5 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | 8-12% PEG4K, 100mM NaAcetate, 100mM Hepes pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |