3N40
Crystal structure of the immature envelope glycoprotein complex of Chikungunya virus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-10-14 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.740, 90.010, 179.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.100 - 2.170 |
R-factor | 0.209 |
Rwork | 0.208 |
R-free | 0.23900 |
Structure solution method | MAD+MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ala |
RMSD bond length | 0.007 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | BUSTER (2.9.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 2.230 |
High resolution limit [Å] | 2.170 | 2.170 |
Number of reflections | 51008 | |
<I/σ(I)> | 10.7 | 2 |
Completeness [%] | 99.5 | 98 |
Redundancy | 4.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | 8-12% PEG4K, 100mM NaAcetate, 100mM Hepes pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |