3N3W

2.2 Angstrom Resolution Crystal Structure of Nuclease Domain of Ribonuclase III (rnc) from Campylobacter jejuni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97965
Spacegroup name	C 2 2 21
Unit cell lengths	62.099, 98.252, 118.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 2.205
R-factor	0.17922
Rwork	0.176
R-free	0.23371
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a11
RMSD bond length	0.013
RMSD bond angle	1.295
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.118	0.513
Number of reflections	18659
<I/σ(I)>	16.7	3.3
Completeness [%]	99.9	99.1
Redundancy	7.3	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	Protein solution: 7.6 mg/mL, 0.5M Sodium chloride, 0.01M Tris pH 8.3 Screen solution: Classics II (F7), 0.2M Ammonium sulfate, 0.1M BIS-TRIS pH 6.5, 25% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K