3N3E
Zebrafish AlphaA crystallin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 33.297, 33.297, 354.337 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.615 - 1.750 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l1e |
RMSD bond length | 0.007 |
RMSD bond angle | 1.108 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
Rmerge | 0.063 | 0.041 | 0.344 |
Number of reflections | 21407 | ||
<I/σ(I)> | 11.4 | ||
Completeness [%] | 98.2 | 90.9 | 99.6 |
Redundancy | 7 | 5.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M MIB pH 6.0, 25% PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 298K |