3N3D
Crystal structure of geranylgeranyl pyrophosphate synthase from lactobacillus brevis atcc 367
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-23 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 111.562, 111.562, 198.497 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.235 |
Rwork | 0.234 |
R-free | 0.26700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.118 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 49591 | |
<I/σ(I)> | 8.2 | 1 |
Completeness [%] | 98.7 | 99.9 |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.1M TRIS-HCL, PH 7.0, 2M AMMONIUM SULFATE, 200MM LITHIUM SULFATE, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |