3N39
Ribonucleotide Reductase Dimanganese(II)-NrdF from Escherichia coli in Complex with NrdI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2009-12-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0781 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.403, 90.721, 143.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.840 - 2.500 |
R-factor | 0.237 |
Rwork | 0.235 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3N37 and 1RLJ |
RMSD bond length | 0.006 |
RMSD bond angle | 0.856 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.840 |
High resolution limit [Å] | 2.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.6 | 20% PEG 3000, 0.1 M HEPES pH 7.6, 0.1 M lithium sulfate |