3N30
Crystal Structure of cubic Zn3-hUb (human ubiquitin) adduct
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.282 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 104.890, 104.890, 104.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.169 - 3.000 |
R-factor | 0.2376 |
Rwork | 0.234 |
R-free | 0.31770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.324 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | ILMILIONE (v.2.2) |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.169 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.063 | 0.508 |
Number of reflections | 4319 | |
<I/σ(I)> | 40.1 | 6.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 25 mg/ml milliQ water protein solution, 25% (w/v) PEG 1450, 50 mM HEPES, 200 mM Zn acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |