3N2D
Crystal Structure of the Complex of type I Ribosome inactivating protein with hexapeptide Ser-Asp-Asp-Asp-Met-Gly at 2.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5414 |
| Spacegroup name | H 3 |
| Unit cell lengths | 131.186, 131.186, 40.734 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.840 - 2.220 |
| R-factor | 0.18608 |
| Rwork | 0.183 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aha |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.534 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.700 | 2.290 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 12968 | |
| <I/σ(I)> | 12.6 | 2 |
| Completeness [%] | 99.2 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 14% PEG 6000, 0.1M Sodium Phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
