3N27
Molecular Basis of the Inhibition of Henipa Viruses
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97893 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.392, 54.055, 134.126 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.100 - 1.800 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.24207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wp8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.206 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.100 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.427 |
| Number of reflections | 20844 | |
| <I/σ(I)> | 13.7 | 2.6 |
| Completeness [%] | 92.5 | 75.3 |
| Redundancy | 5.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | tert-Butanol 35% (v/v), sodium citrate 0.1M, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
