3N1N
Crystal structure of the complex of type I ribosome inactivating protein with guanine at 2.2A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 292 |
Detector technology | IMAGE PLATE |
Collection date | 2009-06-22 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | H 3 |
Unit cell lengths | 131.640, 131.640, 41.210 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.950 - 2.230 |
R-factor | 0.173 |
Rwork | 0.173 |
R-free | 0.19500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aha |
RMSD bond length | 0.009 |
RMSD bond angle | 1.900 |
Data reduction software | AUTOMAR |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.250 |
High resolution limit [Å] | 2.230 | 2.230 |
Number of reflections | 12622 | |
Completeness [%] | 97.8 | 94.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Phosphate buffer, PEG6000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |