3N1J

Crystal structure of a StWhy2-dT32 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1.08
Spacegroup name	F 4 3 2
Unit cell lengths	166.495, 166.495, 166.495
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.197 - 2.650
R-factor	0.216
Rwork	0.211
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l3a
RMSD bond length	0.006
RMSD bond angle	0.997
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.740
High resolution limit [Å]	2.650	2.650
Number of reflections	6188
<I/σ(I)>	9.1	3
Completeness [%]	100.0	100
Redundancy	19.1	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	25% PEG6000, 0.1M Tris, 1.2M LiCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K