3N1G
Crystal structure of DhhN bound to BOCFn3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | RIGAKU SATURN 944+ |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 87.210, 92.985, 74.684 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.648 - 1.900 |
R-factor | 0.1842 |
Rwork | 0.182 |
R-free | 0.22180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.140 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 47802 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 298 | 30% PEG 4000, 200 mM MgCl2, 100 mM Tris, pH 8.0, VAPOR DIFFUSION, temperature 298K |