3N1B
C-terminal domain of Vps54 subunit of the GARP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.700 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.862, 30.323, 88.953 |
Unit cell angles | 90.00, 120.50, 90.00 |
Refinement procedure
Resolution | 30.976 - 2.398 |
R-factor | 0.241 |
Rwork | 0.239 |
R-free | 0.28700 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.547 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RESOLVE (2.13) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.398 | 5.170 | 2.400 |
Rmerge | 0.066 | 0.061 | 0.200 |
Number of reflections | 11565 | ||
<I/σ(I)> | 18.4 | ||
Completeness [%] | 98.4 | 98.6 | 90.3 |
Redundancy | 3.3 | 3.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 23% PEG3350, 0.1M BisTris, 0.1M I3C, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 23% PEG3350, 0.1M BisTris, 0.1M I3C, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |