3N0X

Crystal structure of an ABC-type branched-chain amino acid transporter (RPA4397) from Rhodopseudomonas palustris CGA009 at 1.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97949,0.97898
Spacegroup name	P 1 21 1
Unit cell lengths	58.736, 41.806, 75.979
Unit cell angles	90.00, 110.63, 90.00

Refinement procedure

Resolution	29.348 - 1.500
R-factor	0.149
Rwork	0.148
R-free	0.17200
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.508
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.348	29.348	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.043	0.018	0.441
Number of reflections	55240	10494	9643
<I/σ(I)>	11.69	36.2	1.7
Completeness [%]	96.6	96.5	94.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.2000M NaOAc, 30.0000% PEG-8000, 0.1M Cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K