3N0W
Crystal structure of a branched chain amino acid ABC transporter periplasmic ligand-binding protein (Bxe_C0949) from BURKHOLDERIA XENOVORANS LB400 at 1.88 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-24 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91162,0.97915,0.97898 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.545, 58.269, 145.338 |
| Unit cell angles | 90.00, 98.96, 90.00 |
Refinement procedure
| Resolution | 29.134 - 1.880 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.23300 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.517 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.134 | 29.134 | 1.970 |
| High resolution limit [Å] | 1.880 | 4.080 | 1.900 |
| Rmerge | 0.070 | 0.030 | 0.558 |
| Number of reflections | 62570 | 11992 | 7664 |
| <I/σ(I)> | 6.93 | 18.9 | 1.4 |
| Completeness [%] | 91.9 | 92.2 | 57.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.86 | 293 | 14.0000% polyethylene glycol monomethyl ether 2000, 0.0100M nickel (II) chloride, 0.1M TRIS pH 8.86, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 293K |
