3N0V

Crystal structure of a formyltetrahydrofolate deformylase (PP_0327) from PSEUDOMONAS PUTIDA KT2440 at 2.25 A resolution

Experimental procedure

Experimental method	MAD
Detector technology	CCD
Collection date	2010-02-11
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	81.213, 93.965, 97.129
Unit cell angles	90.00, 101.38, 90.00

Refinement procedure

Resolution	42.133 - 2.250
R-factor	0.225
Rwork	0.222
R-free	0.27400
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.046
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	SHELX
Refinement software	REFMAC (5.6.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.133
High resolution limit [Å]	2.250
Number of reflections	67847
Completeness [%]	99.8
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	0.1860M potassium fluoride 20.4000% polyethylene glycol 3350, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	0.1860M potassium fluoride 20.4000% polyethylene glycol 3350, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	0.1860M potassium fluoride 20.4000% polyethylene glycol 3350, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K