3N0P
A mutant human Prolactin receptor antagonist H30A in complex with the extracellular domain of the human prolactin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0810 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.540, 123.540, 73.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.670 - 2.100 |
| R-factor | 0.18223 |
| Rwork | 0.180 |
| R-free | 0.22388 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mzg |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.049 |
| Data reduction software | MOSFLM (3.3.9) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 106.989 |
| High resolution limit [Å] | 1.787 |
| Number of reflections | 37230 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 3.7M NaCl, 0.1 M Hepes pH 7.5, temperature 291K, VAPOR DIFFUSION, HANGING DROP |
