3N0L
Crystal structure of serine hydroxymethyltransferase from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-02-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.558, 108.319, 126.072 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.300 - 1.800 |
R-factor | 0.162 |
Rwork | 0.160 |
R-free | 0.19800 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.060 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.076 | 0.648 |
Number of reflections | 74111 | |
<I/σ(I)> | 10.1 | 2.2 |
Completeness [%] | 98.6 | 88.5 |
Redundancy | 7.5 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% PEG 1500, 200mM NH4 Sulfate, 100mM Bis-Tris, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |