3N0B
TM0449 mutant crystals grown in loops/micromounts
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-10 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.523, 116.754, 141.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.20028 |
Rwork | 0.198 |
R-free | 0.24884 |
Structure solution method | Rigid body refinement |
Starting model (for MR) | 1o26 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.843 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.600 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.102 | 0.790 |
Number of reflections | 40897 | |
<I/σ(I)> | 11.1 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.6 | 295 | 49% PEG 200, 0.1M HEPES (pH 7.6), grown in loops by a new method, temperature 295K |