3N06
A mutant human Prolactin receptor antagonist H27A in complex with the extracellular domain of the human prolactin receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0809 |
Spacegroup name | P 65 |
Unit cell lengths | 123.419, 123.419, 72.665 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.857 - 2.000 |
R-factor | 0.19704 |
Rwork | 0.195 |
R-free | 0.24051 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mzg |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.000 | 31.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.131 | 0.063 | 0.940 |
Number of reflections | 42787 | ||
<I/σ(I)> | 8.5 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 17.5 | 21.7 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 3.6M NaCl, 0.1 M Hepes 7.5, temperature 291K, VAPOR DIFFUSION, HANGING DROP |