3MZQ
RNase crystals grown by the hanging drop method
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.240, 64.240, 63.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.17665 |
| Rwork | 0.176 |
| R-free | 0.19335 |
| Structure solution method | Rigid body refinement |
| Starting model (for MR) | Structure refinement using the data collected for the loop method (used PDB code: 3DH5 for MR for the loop method) |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.702 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.046 | 0.446 |
| Number of reflections | 24750 | |
| <I/σ(I)> | 23.63 | 3.9 |
| Completeness [%] | 99.8 | |
| Redundancy | 5.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 1.7M Ammonium sulfate, 2M NaCal, 0.1M Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
