3MZI
Crystallographic structure of the pseudo-Signaling State of the BLUF Photoreceptor PixD (slr1694) Y8F mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-01 |
Detector | NOIR-1 |
Wavelength(s) | 1.240 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.850, 119.480, 93.260 |
Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
Resolution | 49.925 - 2.304 |
R-factor | 0.1665 |
Rwork | 0.165 |
R-free | 0.21550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2HFN; chain A |
RMSD bond length | 0.007 |
RMSD bond angle | 1.241 |
Data reduction software | XDS |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.060 | 0.217 |
Number of reflections | 37424 | |
<I/σ(I)> | 13.08 | 4.49 |
Completeness [%] | 53.9 | 8 |
Redundancy | 3.7 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.1 M (NH4)2SO4, 0.1 M Bis-Tris, 0.01 M Spermidine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |