3MYW
The Bowman-Birk type inhibitor from mung bean in ternary complex with porcine trypsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 283 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1990-07-16 |
| Detector | ENRAF-NONIUS FAST |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 |
| Unit cell lengths | 62.460, 62.460, 160.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pdb |
| Data reduction software | PROTEIN |
| Data scaling software | PROTEIN |
| Phasing software | PROTEIN |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 10516 | |
| Completeness [%] | 77.5 | 35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.5M ammonium sulfate, 0.2M sodium potassium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
