3MYK
Insights into the Importance of Hydrogen Bonding in the Gamma-Phosphate Binding Pocket of Myosin: Structural and Functional Studies of Ser236
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97885 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.005, 147.021, 153.425 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.910 - 1.840 |
R-factor | 0.19458 |
Rwork | 0.193 |
R-free | 0.23009 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vom |
RMSD bond length | 0.026 |
RMSD bond angle | 2.045 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.910 | 1.910 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.051 | 0.152 |
Number of reflections | 84770 | |
<I/σ(I)> | 35.7 | 5.3 |
Completeness [%] | 98.7 | 97.6 |
Redundancy | 6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 12% PEG8K, 250mM MgCl2, 100mM MOPS, 2mM AMPPNP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |