3MY7
The Crystal Structure of the ACDH domain of an Alcohol Dehydrogenase from Vibrio parahaemolyticus to 2.25A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.654, 145.489, 91.986 |
| Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.232 |
| Rwork | 0.229 |
| R-free | 0.28100 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.373 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.084 | 0.547 |
| Number of reflections | 79755 | |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 277 | 30% PEG 400, 0.1M Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
