3MY6
Crystal Structure of the complex of type 1 ribosome inactivating protein with 7-methylguanine at 2.65 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 292 |
Detector technology | IMAGE PLATE |
Collection date | 2009-07-21 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | H 3 |
Unit cell lengths | 131.428, 131.428, 39.808 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.760 - 2.650 |
R-factor | 0.174 |
Rwork | 0.174 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aha |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.750 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 7355 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | PEG, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |