3MY2
Crystal structure of LptC
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-05-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9764 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 94.210, 94.210, 60.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.000 - 2.200 |
R-factor | 0.19606 |
Rwork | 0.195 |
R-free | 0.22226 |
Structure solution method | SAD |
RMSD bond length | 0.025 |
RMSD bond angle | 2.056 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.084 | 0.604 |
Number of reflections | 14109 | |
<I/σ(I)> | 15.1 | 2.3 |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 6.7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.04 M K2SO4, 16% PEG8000, 20% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |