3MY2
Crystal structure of LptC
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-05-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 94.210, 94.210, 60.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 2.200 |
| R-factor | 0.19606 |
| Rwork | 0.195 |
| R-free | 0.22226 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.056 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.084 | 0.604 |
| Number of reflections | 14109 | |
| <I/σ(I)> | 15.1 | 2.3 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.04 M K2SO4, 16% PEG8000, 20% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
