3MXQ

Crystal structure of sensory box sensor histidine kinase from Vibrio cholerae

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97940
Spacegroup name	C 1 2 1
Unit cell lengths	58.237, 102.202, 124.969
Unit cell angles	90.00, 100.12, 90.00

Refinement procedure

Resolution	50.000 - 2.780
R-factor	0.22724
Rwork	0.225
R-free	0.25743
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.218
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.780	2.800
Rmerge	0.102	0.754
Number of reflections	18036
<I/σ(I)>	43.5	2.67
Completeness [%]	99.8	99.7
Redundancy	9.3	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	297	0.1M Bis-Tris, 1.2 M Na/K tartrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K
1	VAPOR DIFFUSION, SITTING DROP	7	297	0.1M Bis-Tris, 1.2 M Na/K tartrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K