3MXN
Crystal structure of the RMI core complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Detector technology | CCD |
Collection date | 2009-07-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97933, 0.97856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.096, 42.223, 152.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.800 - 1.550 |
R-factor | 0.1972 |
Rwork | 0.195 |
R-free | 0.23106 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.191 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 39281 | |
<I/σ(I)> | 25.7 | 2.4 |
Completeness [%] | 97.0 | 83 |
Redundancy | 10.8 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 10 mM Hepes pH 7.1, 0.05 M NaSCN, 20% ethylene glycol, 2 mM DTT, 13% polyethylene glycol 3350, with benzamidine (1%) added to the drop, VAPOR DIFFUSION, HANGING DROP, temperature 298K |