3MXL
Crystal structure of nitrososynthase from Micromonospora carbonacea var. africana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.200 |
| Spacegroup name | P 65 |
| Unit cell lengths | 103.815, 103.815, 295.954 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.150 |
| R-factor | 0.2457 |
| Rwork | 0.242 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jif |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.144 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.474 | |
| Number of reflections | 29783 | |
| <I/σ(I)> | 12.8 | 2.5 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris-HCl, 0.2M MgCl2, 10% PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
