3MX7
Crystal Structure Analysis of Human FAIM-NTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-16 |
Detector | SBC-2 |
Wavelength(s) | 1.04361 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 57.170, 57.170, 69.946 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.570 - 1.760 |
R-factor | 0.17759 |
Rwork | 0.176 |
R-free | 0.20045 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.741 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.069 | 0.187 |
Number of reflections | 14391 | |
<I/σ(I)> | 17.267 | 11.041 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 5.7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.67M sodium phosphate, 0.73M potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |