3MX2
Lassa fever virus Nucleoprotein complexed with dTTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-01-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 3 |
| Unit cell lengths | 176.147, 176.147, 56.474 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 152.548 - 1.983 |
| R-factor | 0.1818 |
| Rwork | 0.180 |
| R-free | 0.20806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mwp |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.587 |
| Data reduction software | MOSFLM |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 152.548 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.074 | 0.565 |
| Number of reflections | 134680 | |
| <I/σ(I)> | 11.9 | 2.6 |
| Completeness [%] | 97.0 | 95.8 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG3350, 0.2 M Lithium chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG3350, 0.2 M Lithium chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
