3MWZ
Crystal structure of the selenomethionine derivative of the L 22,47,100 M mutant of sialostatin L2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97120 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 95.267, 95.267, 95.267 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.420 - 1.520 |
| R-factor | 0.17568 |
| Rwork | 0.174 |
| R-free | 0.20136 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.219 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.420 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.080 | 0.375 |
| <I/σ(I)> | 14.5 | 6.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 15 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 1.8 M ammonium sulfate, 0.1 M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP |
