3MWY

Crystal structure of the chromodomain-ATPase portion of the yeast Chd1 chromatin remodeler

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792,0.9611
Spacegroup name	P 61 2 2
Unit cell lengths	94.333, 94.333, 450.088
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.700
R-factor	0.26462
Rwork	0.262
R-free	0.31772
Structure solution method	MAD
Starting model (for MR)	PDB ENTRIES 2H1E 1z63 1z3i
RMSD bond length	0.015
RMSD bond angle	1.628
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0091)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.830
High resolution limit [Å]	3.700	3.700
Rmerge	0.069	0.402
Number of reflections	20114
<I/σ(I)>	20.9	4.5
Completeness [%]	99.9	99.8
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	18% PEG 3350; 400 mM K+/Na+ tartrate; 5% xylitol; 10 mM MgCl2; 1 mM ATPgammaS, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K