3MWR

Crystal structure of ribonuclease A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-07-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	101.903, 33.067, 73.869
Unit cell angles	90.00, 90.39, 90.00

Refinement procedure

Resolution	24.033 - 1.850
R-factor	0.177
Rwork	0.175
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mwq
RMSD bond length	0.003
RMSD bond angle	0.808
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.21)
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.922	24.630	1.950
High resolution limit [Å]	1.850	5.850	1.850
Rmerge	0.080	0.055	0.558
Total number of observations		3422	14884
Number of reflections	21141
<I/σ(I)>	5.804	10	1.3
Completeness [%]	98.6	94.1	97.3
Redundancy	5	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	286	30% PEG 8000, 0.2M ammonium sulfate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 286K