3MWR
Crystal structure of ribonuclease A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.903, 33.067, 73.869 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 24.033 - 1.850 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mwq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.808 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.922 | 24.630 | 1.950 |
| High resolution limit [Å] | 1.850 | 5.850 | 1.850 |
| Rmerge | 0.080 | 0.055 | 0.558 |
| Total number of observations | 3422 | 14884 | |
| Number of reflections | 21141 | ||
| <I/σ(I)> | 5.804 | 10 | 1.3 |
| Completeness [%] | 98.6 | 94.1 | 97.3 |
| Redundancy | 5 | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 286 | 30% PEG 8000, 0.2M ammonium sulfate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
