3MWQ
Crystal structure of ribonuclease A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-07-07 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.632, 32.915, 73.391 |
Unit cell angles | 90.00, 91.03, 90.00 |
Refinement procedure
Resolution | 24.460 - 1.680 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1srn |
RMSD bond length | 0.004 |
RMSD bond angle | 0.909 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.21) |
Phasing software | AMoRE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 73.379 | 24.460 | 1.740 |
High resolution limit [Å] | 1.650 | 5.220 | 1.650 |
Rmerge | 0.055 | 0.049 | 0.282 |
Total number of observations | 4392 | 7772 | |
Number of reflections | 27506 | ||
<I/σ(I)> | 5.949 | 9.6 | 2.7 |
Completeness [%] | 92.6 | 99.2 | 57.4 |
Redundancy | 4.3 | 4.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 286 | 30% PEG 8000, 0.2M ammonium sulfate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 286K |