3MWL
Q28E mutant of HERA N-terminal RecA-like domain in complex with 8-OXOADENOSINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.178, 92.178, 108.975 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.906 - 1.600 |
R-factor | 0.1976 |
Rwork | 0.196 |
R-free | 0.23640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gxs |
RMSD bond length | 0.006 |
RMSD bond angle | 1.107 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.906 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.028 | 0.590 |
Number of reflections | 60859 | |
<I/σ(I)> | 13.2 | 1.1 |
Completeness [%] | 97.3 | 70.1 |
Redundancy | 8.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |