3MW6
Crystal structure of NMB1681 from Neisseria meningitidis MC58, a FinO-like RNA chaperone
Replaces: 2HXJExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97932 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.479, 76.527, 122.715 |
Unit cell angles | 90.00, 116.84, 90.00 |
Refinement procedure
Resolution | 42.889 - 2.209 |
R-factor | 0.1929 |
Rwork | 0.191 |
R-free | 0.23480 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.088 | 0.464 |
Number of reflections | 96019 | |
<I/σ(I)> | 26.2 | 1.92 |
Completeness [%] | 97.1 | 79.2 |
Redundancy | 4.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 100MM BIS-TRIS, 170MM MGCL2, 21% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |