3MW3
Crystal structure of beta-neurexin 2 with the splice insert 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.657, 102.087, 78.512 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.250 - 2.330 |
| R-factor | 0.19395 |
| Rwork | 0.191 |
| R-free | 0.25248 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3BOP |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.555 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.350 |
| Rmerge | 0.088 |
| Number of reflections | 8885 |
| <I/σ(I)> | 28.8 |
| Completeness [%] | 96.7 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 22% PEG3350, 0.35M calcium chloride, 1mM GSH/GSSG, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
