3MVK
The Crystal Structure of FucU from Bifidobacterium longum to 1.65A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.047, 83.859, 144.813 |
| Unit cell angles | 90.00, 95.11, 90.00 |
Refinement procedure
| Resolution | 40.720 - 1.650 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.20500 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.338 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.085 | 0.060 | 0.579 |
| Number of reflections | 189426 | ||
| <I/σ(I)> | 8 | ||
| Completeness [%] | 99.9 | 99.6 | 100 |
| Redundancy | 3.3 | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 25% PEG 3350, 0.1M Hepes pH 7.5, 0.2M Sodium chloride, VAPOR DIFFUSION, temperature 298K |
