3MV3
Crystal Structure of a-COP in Complex with e-COP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97960 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 328.126, 74.310, 96.396 |
Unit cell angles | 90.00, 101.98, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.250 |
Rwork | 0.241 |
R-free | 0.28700 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.630 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.370 |
High resolution limit [Å] | 3.250 | 3.250 |
Number of reflections | 62887 | |
<I/σ(I)> | 1.8 | |
Completeness [%] | 100 | |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 100 mM HEPES, pH 7.2 8 % (w/v) tacsimate 100 mM sodium malonate 200 mM sodium chloride 20 % (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |