3MUS

2A Resolution Structure of Rat Type B Cytochrome b5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	297
Detector technology	DIFFRACTOMETER
Collection date	1997-06-20
Detector	SIEMENS FOUR-CIRCLE
Spacegroup name	P 21 21 21
Unit cell lengths	46.260, 71.130, 72.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.176
Rwork	0.176
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1awp
RMSD bond length	0.017
RMSD bond angle	1.580
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.300
High resolution limit [Å]	1.980
Rmerge	0.063
Number of reflections	14715
Completeness [%]	97.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8	277	30%PEG8K, 0.2M MgAc, 0.1M Hepes, pH 6.8, EVAPORATION, temperature 277K