3MUO
APPEP_PEPCLOSE+PP closed state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-26 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 107.770, 107.770, 147.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.280 - 1.950 |
R-factor | 0.16 |
Rwork | 0.160 |
R-free | 0.18300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iuj |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 73036 | |
<I/σ(I)> | 17.3 | 8.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.2 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 8MG/ML PROTEIN IN 20MM HEPES (PH7.5), 100MM NACL, 5% W/V GLYCEROL, AND 1MM EDTA. EQUAL VOLUME OF PROTEIN AND PRECIPITANT (1.5M AMSO4, 100MM TRIS (PH 8.5) AND 12% W/V GLYCEROL) WERE EQUILIBRATED BY VAPOR DIFFUSION AT 14C. CRYOSOLUTION IS 2.2M AMSO4, 30% W/V SUCROSE, 12% W/V GLYCEROL AND 100MM TRIS (PH 8.5). CRYSTALS WERE SOAKED WITH 10MM OF THE INHIBITOR ZPR IN CRYOSOLUTION. |