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3MUC

MUCONATE CYCLOISOMERASE VARIANT I54V

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]298
Detector technologyIMAGE PLATE
DetectorRIGAKU RAXIS IIC
Spacegroup nameI 4
Unit cell lengths139.600, 139.600, 84.200
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution8.000 - 2.300
R-factor0.176
Rwork0.176
R-free0.22300
Structure solution methodOTHER
RMSD bond length0.009
RMSD bond angle1.500
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Refinement softwareX-PLOR (3.851)
Data quality characteristics
 Overall
Low resolution limit [Å]8.000

*

High resolution limit [Å]2.300
Rmerge0.121
Total number of observations132958

*

Number of reflections30586

*

Completeness [%]80.2
Redundancy4.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1double dialysis

*

6.5pH 6.5
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
111MES20 (mM)
2112 (mM)
31150 (mM)
4110.02 (%(w/v))
511beta-mercaptoethanol7 (mM)
611protein8 (mg/ml)

221051

PDB entries from 2024-06-12

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