3MTS
Chromo Domain of Human Histone-Lysine N-Methyltransferase SUV39H1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97944 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 99.528, 99.528, 118.321 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.830 - 2.200 |
| R-factor | 0.21005 |
| Rwork | 0.208 |
| R-free | 0.24379 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kna |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.290 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.2.3) |
| Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.084 | 0.044 | 0.502 |
| Number of reflections | 11662 | ||
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 9.3 | 8.8 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 3.5M Na Formate, 0.1M Bis-Tris Propane pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
