3MTI
The Crystal Structure of a rRNA Methylase from Streptococcus thermophilus to 1.95A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.331, 57.044, 75.365 |
Unit cell angles | 90.00, 109.11, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.950 |
R-factor | 0.24 |
Rwork | 0.238 |
R-free | 0.27200 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.360 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
Rmerge | 0.085 | 0.066 | 0.578 |
Number of reflections | 28265 | ||
<I/σ(I)> | 8.1 | ||
Completeness [%] | 99.5 | 96.9 | 99.9 |
Redundancy | 3.3 | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 25% PEG 3350, 0.1M HEPES pH 7.5, 0.2M Sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |