3MTE
Crystal Structure of 16S rRNA Methyltranferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.651, 59.816, 91.697 |
| Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
| Resolution | 36.250 - 1.805 |
| R-factor | 0.1738 |
| Rwork | 0.173 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mqh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.027 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.300 | 1.860 |
| High resolution limit [Å] | 1.805 | 1.805 |
| Rmerge | 0.067 | 0.403 |
| Number of reflections | 49392 | |
| <I/σ(I)> | 20 | 3.4 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 14% PEG 6000, 4% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
