3MSV

The hypoxic regulator of sterol synthesis Nro1 is a nuclear import adaptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-20
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	47.790, 107.960, 100.780
Unit cell angles	90.00, 102.60, 90.00

Refinement procedure

Resolution	19.730 - 2.180
R-factor	0.2072
Rwork	0.205
R-free	0.25701
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.250
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.730	2.300
High resolution limit [Å]	2.180	2.180
Rmerge		0.515
Number of reflections	51200
<I/σ(I)>	12.9
Completeness [%]	98.5	93.2
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1.3 M ammonium tartrate dibasic, 0.1 M Bis-TrisHCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 6.5
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1.3 M ammonium tartrate dibasic, 0.1 M Bis-TrisHCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 6.5